Does anyone have any experience in assaying intracellular
benzyloxycarbonyl tetra peptide AFC (7-Amino-4-Trifluoromethyl
Coumarin substrates.A few simple questions follow.
1 Are these compounds generally cell permeable
2 Can the cleaved AFC be excited at 352 nm , if so what is
the emission wavelength.
3 Does the cleaved AFC diffuse out of the cell.
Thanks for any help
Roger Snowden
rts1@le.ac.uk
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web