He would like your advice on the best protocols to achieve this.
Do the new "fix and perm" type reagents adequately permeabilize
the nuclear membrane?
Have there been major advances in this area since the older
protocols designed for total nuclear DNA measurements?
Any advice would be gratefully received, and if there is interest
we will post a summary of responses on this mailing list.
Thanks,
Ken Ault aultk.mmcri@office.mmc.org
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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