quantifying mitochondria -Reply
mohedley@spiff.pmh.toronto.on.ca
Thu, 21 Mar 1996 10:18:51 -0500
We have some experience labelling mitochondria with dodecyl acridine
orange. This is obtainable from Molecular Probes, has similar spectra to
FITC, and labels live cells. Unlike rhodamine 123 it is probably not
dependent on mitochondrial membrane potential, and might therefore give
a more accurate measurement of the number of mitochondria per cell. I
have no idea why it should be specific for mitochondria, but when we did
some confocal work in Paul Robinson's lab this appeared to be the case.
There are references to this compound in the Molecular Probes
catalogue.
David Hedley
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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