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I have a researcher interested in studying naive vs. memory T cells. It is
my understanding that naive T cells are CD45RO-/CD45RA+, and that memory T
cells are CD45RO+/CD45RA-. Is this consistent with current experience? If
so, what is the source of a pair of antibodies that work well for this
discrimination? Is a 2-color determination sufficient, or is a 3rd or 4th
marker needed?
How about naive vs. memory B cells? Does CD45RO/RA work for these as well,
or is CD44 better?
Thanks in advance.
* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
* Richard K. Meister Email: Meister.1@osu.edu *
* The Ohio State University Voice: (614) 292-9716 *
* Dept. of Veterinary Biosciences FAX: (614) 292-6473 *
* Cytometry Instrumentation Lab *
* 1925 Coffey Road *
* Columbus, OH 43210 U.S.A. *
* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web