The PerCP beads allow compensation to be consistent, convenient and
easy (especially if you have the newest FACSComp on a FACStation), but
can't solve the FL1 overlap.
Dennis
*****************************************************************************
* Dennis J. Young Voice : (619)
822-0407 * * Flow Cytometry Core Facility FAX :
(619) 822-0412 * * University of California, San Diego USA
e-mail: djyoung@ucsd.edu *
*****************************************************************************
______________________________ Reply Separator
_________________________________ Subject: PerCP Author:
zinetti@acpub.duke.edu at @UCSD Date: 5/9/96 4:55 PM
Paul J Jansen here,
In the past we have used cychrome (??spell??) as a "third"
color on our one laser Facscan. The problem we had with it was
difficult to compensate between FL1 and FL3. We are considering using
PerCP now and I would like to know if anyone has experience with it.
BD has promised to throw in calibrite beads capable of doing the three
color compensation. Comments? Questions? Suggestions? Thanx Paul J
Jansen zinetti@acpub.duke.edu http://www.duke.edu/~zinetti DUMC
Immunology http://www.duke.edu/~zinetti/Immunology/immuno1.html
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>MIME-Version: 1 >Content
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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