pH measurements

Ken McDonald (kmcdonald@microimm.mcgill.ca)
Fri, 11 Apr 1997 11:35:37 -0500

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Hi Flowers..I have a customer who is interested in measuring pH changes
in cells in relation to apoptosis.The paper he has shown me is from JBC
vol. 271, no 27 (pp 16260-16262) 1996.The paper refers to a method
called radiometric flow cytometry.We definitely don't want to do this
with our machine(Facs Vantage).I've heard there is a method using ratio
measurements(ie fl2/fl3) in which pH measurments can be made. Can anyone
give me any more details about this. I thank you in advance.
Ken McDonald

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu