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We are trying to quantitate surface marker changes simultaneously with DNA
cell cycle analysis (using PI). We have tried this using ethanol fixation,
but have lost the surface markers.
Can anyone suggest a recipe for a better fixation method?
Thanks all!
Lyndal Russell
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web