pulse processing in cell cycle analysis

Fang-Yao Hou (FYHOU@macc.wisc.edu)
Tue, 06 May 97 18:34 CST

Dear FLOW experts:

This is to ask your opinions about using FL-height, instead of FL-area in
cell cycle analysis. I can see typical picture of G1, S and G2/M from FL-
height histogram as long as I acquire data in linear mode. I am wondering
if this is an acceptable way to do cell cycle analysis without purchasing
the pulse processing module. The cytometer I am using is the BD Vantage.
I appreciate your responses.

Fang-Yao Hou
FYHOU@macc.wisc.edu


Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu