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I recently had to interprete a lymphocyte transformation/activation
assay on a 22 year female with history of repeated pulmonary
infections. All the usual lymphocyte subset, immunoglobulin and
complement assays were normal. However our assay for PHA and ConA (not
PWM) induced CD69 expression for T cells (not B cells) was below our
normal range, yet the proliferative response to the same antigens, as
measured by BRDU incorporation and %S phase, was normal. I have not
seen this before, nor able to find anything on a literature search. Has
anyone had or know of a similiar experience?
Bruce H. Davis
Wm. Beaumont Hospital
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web