Re: 7-AAD use with plant nuclei

N.W. BLACKHALL (PLZNB@pln1.life.nottingham.ac.uk)
Thu, 29 May 1997 08:47:35 GMT0BST

Dear all,

In her article of May 28th, Cindy Gross wrote:

> I am now interested in trying to substitute another DNA
> stain, 7-AAD, for the propidium iodide.

To me this sounds an unusual choice, although I can think of several
reasons for using fluorochromes other than PI.

Perhaps people would be kind enough to enlighten me of the special
properties of 7-AAD.

Thank you

Nigel Blackhall
University of Nottingham, UK
Nigel.Blackhall@Nottingham.ac.uk


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