pH measurements
Ken McDonald (KMCDONALD@MICROIMM.MCGILL.CA)
Mon, 02 Jun 1997 15:55:00 -0500
Hi Flowers,
My question concerns pH measurment using Snarf and the ratio setup. I
know that with calcium flux (using indo-1) that your settings have to be
on linear (gain of 1) for fl4 and fl5(using the facs Vantage).Is this
also the case for pH ratio measurements which also use a ratio for fl2
and fl3. Could I play around with the gains instead of leaving them at
gain 1 for fl2 and fl3.
Thank you in advance...Ken McDonald
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
