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Is anyone familiar with a problem of a fluorescence signal from beads
falling down over a course of few hours/a day on B-D FACSort analyzer?
Here are the details:
- We measure an Fl1 signal from FCS beads with 15.8K FITC in linear mode.
First thing in the morning the mean value read for the beads is ~ 550 on a
scale of 1024 ( laser was warmed up for 30 min).
- Beads are always in PBS 0.1% BSA and for every measurement we use fresh
beads. Beads are not expired.
- Every hour a signal drops 10-20 channels, so at the end of the day the
difference is ~80-160 channels.
- All "Instrument status" values are looking good and constant.
I have been experiencing this problem for a few months. In the mean time
the instrument underwent maintenance service , exchange of a board with FL1
electronics, cleaning etc..
"The problem" makes my life a little difficult so the question is how to get
rid of it. One can suggest a reward together with the solution ( if it works!).
Thanks for taking your time.
Visia
Visia Dragowska
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web