Viscosity Effects on FCM Analysis
Matthew J Shaw (shawmj@battelle.org)
Mon, 16 Jun 1997 08:17:33 -0400 (EDT)
All,
Does anyone have a good feel for the effects of higher viscosity
fluids being analyzed via flow cytometric means? For example, the
viscosity of water is 1 centipoise, and I assume that cells that are
within water can be reliably analyzed. I believe that the viscosity
of blood is perhaps 3 centipoise; again, I assume that cells within
this matrix can be reliably analyzed. However, if the matrix is say
10 centipoise, there may be a problem with forcing the matrix through
the FCM nozzle. Maybe a problem doesn't exist until the matrix is 100
centipoise? Has anyone done a study like this on any flow cytometer,
or have a good feel for this effect? What is the upper viscosity
limit before any problems may occur? (I realize that one could dilute
the sample with a low-viscosity liquid until the desired viscosity is
reached - I'd rather not have to do that!) I would appreciate any help
on this matter.
Matt Shaw
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
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If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
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