INDO-1 Calibration

HANDLEY@sorter.dfci.harvard.edu
Mon, 16 Jun 1997 10:27:13 -0400 (EDT)

Hello Everyone!

Does anyone know if there is a kit available to help calibrate a flow cytometer
for calcium flux using Indo-1? I have a researcher who wants to be able to
compare values between experiements, and I have suggested using ionophore
and EGTA to help with this, but he seems reluctant to do all the calculations
involved. Any suggestions?

Thanks,
Maris

Maris Handley
Dana-Farber Cancer Institute
Boston, Ma 02115
(617)632-3179

handley@sorter.dfci.harvard.edu


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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu