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I have been looking into the economics of our flow setup and discovered =
that I can buy PECy5 anti CD45 for much cheaper than I currently buy =
PerCP anti CD45. I proceeded to make some three color CD3/4/45 and =
3/4/45 PBL's to do some compensation with and found that I got into real =
trouble :(
Did something drastic go wrong with the labeling of the PBL's ??, or is =
there something I need to know about PE-Cy5 on a Calibur (ie: has anyone =
else done this easily/with difficulty ??)
One problem is that I have very little quality information on the =
Calibur - the manual seems to make no mention of the of exactly which =
mirrors/filters the Calibur uses ... does it use a 650LP filter on the =
FL3 detector the same as a FacsScan does ?? (if that's the case then I'm =
only just picking up the *tail* of the PE-Cy5 emission)
Peter Chapple
Melbourne AUSTRALIA
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web