bulk removal of dead cells

Ralph Bohmer (ralph.bohmer@es.nemc.org)
Thu, 19 Jun 97 10:15:33 EDT

Is there any perfect method to bulk isolate a small number of vital cells
from a much larger population of dead and dying cells (apoptotic, necrotic,
debris) ?

It would have to be quantitative.

There is lysis of dead cells by protease. Does that safely preserve all
viable cells ? If yes, what are the best conditions ?

I was told it can be done by density gradient, but I cannot figure out how
that should work.

Many thanks for your opinions.

Ralph M. Bohmer


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