PI & FITC ON THE ELITE
You do not say which configuation you are using for your optical
filters. If you are using PI to measure the cell cycle, it is best to
set the discriminator on red fluorescence. This would usually be on
PMT4 but depends on the filter layout.
If you are using PI to discriminate between dead and live cells,
you should trigger on forward scatter.
Best wishes,
Mike Ormerod
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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