Higher sensitivity
Mario Roederer (ROEDERER@Darwin.Stanford.EDU)
Wed, 02 Aug 1995 09:18:47 -0700 (PDT)
We have successfully used a system devised by Peter Lansdorp up in
Vancouver (at the Terry Fox Cancer Center). He developed an anti-PE
antibody... the basic procedure is to stain cells with and
PE-conjugated antibody to what your interested in. Then, do successive
iterations of staining with biotinylated anti-PE (from Peter), followed
by PE-conjugated avidin. (Several washes between each step!). We
found no significant increase in background staining, and after 3
rounds we had a 5-10 fold amplification of signal.
It's a pretty cute system. For information, please contact him directly.
mr
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
