re: Use of FDA
Michael Fox (MFox@vines.ColoState.EDU)
Wed, 16 Aug 95 11:12:30 MDT
We have used FDA to discriminate between viable and non-viable cells (based
upon membrane integrity). We were using CHO cells but it should work with
virtually any cells that have esterases. You only have to use a very small
amount of FDA since fluorescein is so bright. We add 0.02 ml to 100 ng/ml of
FDA in PBS to 1 ml of a cell suspension in PBS and incubate for 10 min at
room temperature. You can also co-stain with PI which gives the opposite
test of viability - PI is excluded from live cells and FDA (actually the free
fluorescein) is contained in live cells.
Mike Fox
Colorado State University
Ft. Collins, CO
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