To detect apoptotic cells, you might consider using less Hoechst 33342
( 1ugml) for a short time at 37C (5-10 min). In some cells, the apoptotic
cells take up the Hoechst dye more rapidly than the normal. I have
published this method in J. Immun. Meth. and have described in more detail
in Cytometry. A similar method using YOPRO-1 and 488 nm light has just
been published by a French group in J. Immun. Meth. I have tried it and
it worked nicely.
Mike Ormerod
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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