To the UV discussion...200 to 300 mW of UV?!? Fried cells, anyone?
Seriously, I successfully ran my UV output at 30 mW, then used a neutral
density ffilter to reduce the power further to 15 mW (monitored by a
Coherent power meter) to do most of my H33342 viable cell sorting. We
were cconcerned with much with the mutagenic potential of the UV laser, and
wanted to minimize it (given also the mutagenic potential of H33342).
AND still, I saw decent 90 degree scatter in the FACS440.
3) Something I forgot to mention to the plant-people...have they tried
double staining? Using two DNA stains in cobination may improve their
resolution and reduce much of the need to background-correct.
Mark A. KuKuruga
K.C.I.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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