Stephen P. Perfetto
Department of HIV Disease Prevention
Walter Reed Army Institute of Research
1600 East Gide Drive
Rockville, MD. 20850
_______________________________________________________________________________
Subject: detection of mRNA by flow cytometry
From: h-petrie@ski.mskcc.org (Dr. Howard Petrie) at Internet_Gateway
Date: 11/15/95 11:42 AM
Dear fellow cytometrists: I am interested in attempting to detect mRNA on
a single cell basis by in situ staining and flow cytometry. I am aware of
a few papers in the literature (globin message), but they are a little
dates now. I am wondering if anyone could share their experience regarding
similar applications. Are biotinylated riboprobes most likely to be
useful? Which biotinylated nucleotides have been used? How can signal
intensity/ background ratios be maximized? I will repost a summary in the
case of multiple responses. Thanks in advance. HTP
--IMA.Boundary.712035618--
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