PI TOXICITY?

Immuno Flow (Immuno_Flow_at_APPLIC1@ccmail.llu.edu)
Tue, 28 Nov 95 12:29:23 PST

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Please help me win a bet with my boss (I already owe him 3 lunches).
Since the latest discussion about PI staining, we were discussing
protocols and there were a few shall we say disagreements...

1) Is PI toxic to cells?

2) What is the minimum incubation time for PI and cells, say for
live/dead discrimination or DNA?

Thanks (I hope),

Judy the Gambler

The Immunology Center
Loma Linda University Medical Center
11234 Anderson Street, Rm 2573
Loma Linda, CA 92354

(909) 824-0800 Ext. 45376

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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu