Our preliminary results suggest a perturbation of the cell cycle in
transfected cells with GFP, has anyone else seen this?
Any suggestions?
Rochelle Diamond
Caltech Flow Cytometry/Cell Sorting Facility
On Tue, 4 Jun 1996, Ken Hook (313) 996-5746 wrote:
>
> A colleague of mine is trying to transfect cells with a gene that should change
> the cell cycle distribution. She used CD20 cotransfection as a reporter gene
> (with modest separation from non-expressors) but hoped to improve upon that
> with (mutant) GFP. Its expression was disappointing on the FACScan even though
> she could see it under the microscope in unfixed cells. My question is: will
> GFP leak out of cells after we EtOH-fix them for PI staining? If, so, I guess
> I'll have to get the plasma tube on my (too long inactive) HeCd replaced.
>
>
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
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Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web