Although on microscopy they seemed to be a population of fairly large cells
of quite uniform size, on the FSC/SSC plot there seemed to be 2 populations,
one with moderate to high FSC and SSC increasing proportionately,
the other with a lower SSC but an FSC going off the scale.
I wondered if the 2nd lot represented aggregated cells, although she did
trypsinise the cells before fixing them. Both populations had the same
autofluorescence characteristics.
I'd be very grateful for any words of wisdom.
Dr. Dearbhaile O' Donnell
University College Dublin
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
![]() |
![]() |
![]() |
![]() |
![]() |
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web