We have a client who wants to generate a postive control for the TdT
apoptosis assay by treating normal cells with DNAse. I have a few questions
for anyone who has done this.
1. Which type of DNAse would best mimic nucleosomal cleavage?
2. Is it correct to assume that the DNAse treatment should be done after the
cells have been fixed in formaldehyde and ethanol?
3. What concentration, duration, temperature, and buffer should be used?
4. Does it work?
Thanks in advance.
Jeff Clapper
Cell and Hybridoma Facility
Iowa State Univeristy
515-294-8504
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
![]() |
![]() |
![]() |
![]() |
![]() |
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web