Flow Cytometry experience: nine years.
Includes:-
Instruments: FACStar-Plus, with two & three lasers (488nm., U.V.,
pumped dye); Ortho Cytofluorograph 50-H, with twin lasers; FACScan; FACSort;
FACScalibur (prototype); Coulter Epics, Profile & XL.
Data acquisition & analysis computers: Hewlett-Packard 340 (Lysis-II,
CellFit, Paint-a-gate, etc.) & 310 (Facscan Research, Consort-30, Lysis,
etc.); MS-DOS/Windows (WinList, WinMDI, ModFit, IsoContour, etc.); Mac
(ModFit, Mac-Cycle, Paint-a-gate-pro, etc.); VAX; Ortho 2151.
Cell flow-sorting: sterile bulk sorting & single cell cloning (using
Automatic Cell Deposition Unit).
Computer networking: Hewlett-Packard (FACSnet); MS-DOS/Windows/Mac/VAX
(WHPPC, Banyan Vines, Pine, Internet, World Wide Web, etc.).
Training & experience: research, application, development & tuition of
flow cytometry; Establishment, supervision, management & operation of
sophisticated major flow cytometry core facilities, including consultation,
liaision, scheduling & accounting, with direct involvement in many & diverse
research & clinical projects; Field testing of novel reagents & prototype
systems; Flow cytometric techniques, many & varied, especially
immunophenotyping (surface & cytoplasmic), DNA (cell-cycle, apoptosis,
ploidy, etc.), etc. & assorted combinations; Microbiological techniques,
including continuous chemostat culture & system design; Biochemical analyses;
Demonstration for practical classes & student tutorials; Help & supervision
of flow cytometer users, project students & technicians.
Qualifications: Ph.D. Microbiology (1984); B.Sc.(Hons.) Biological
Sciences (1978).
Contact: (214)-648-4076 (work, direct line);
" -369-7564 (home);
E-mail: kruger@utsw.swmed.edu
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web