re: Cryo Preserved Cell Staining

Scott R Burger (burge009@gold.tc.umn.edu)
Fri, 15 Nov 96 15:52:53 -0600

>Anyone with any experience in thawing bags of frozen marrow and keeping
>things alive?
>
>need help
>
>jim houston
>

Sure! We do this in the clinical setting for autologous marrow transplants.
We also do this to obtain hematopoietic progenitor cells and endothelial
cells for more basic research.

Can you describe a bit more specifically what you have in mind? Are there
specific cell populations you want to culture -- stem and progenitor cells,
stromal cells, endothelial cells? How long do want to keep the cells alive?
What will you be doing with the cells?

Scott

***************************************************************
Scott R. Burger, M.D.
Medical Director, Cell Processing Laboratory
University of Minnesota Hospital and Clinic
burge009@gold.tc.umn.edu
612-626-4919
612-624-5411 (Fax)


Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu