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We're looking to isolate nuclei from B lymphocytes and analyse them using
both flow cytometry and 2D electrophoresis. Does anyone have any suggestions
as to how to prepare the nuclei for either method of analysis, or any
references which can point us in the right direction ?
Any help would be much appreciated.
Thanks in advance,
Julia
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Julia Philips
Institute of Dental Research
2 Chalmers St
Surry Hills
NSW 2010
Australia
PH: 61-2-9282 0358
FAX: 61-2-9282 0368
email: jphilips@extro.ucc.su.oz.au
**************************************************
**************************************************
Julia Philips
Institute of Dental Research
2 Chalmers St
Surry Hills
NSW 2010
Australia
PH: 61-2-9282 0358
FAX: 61-2-9282 0368
email: jphilips@extro.ucc.su.oz.au
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web