 |
 |
 |
 |
 |
is there anybody out there doing apoptosis assay by flow to assess cytotoxic
effects on target cells ?
We normally do that by chromium release in a 3 hr assay, but a in special
case we find only few percent specific lysis on target cells even with a
high E:T ratio.
Increasing time of Chromium release assay leads to high spontaneous release
resulting in loss of adequate results.
In our case this poor specific lysis is no basis for further analysis of
specifity of the observed lysis.
What is your experience with apoptosis assay ( Annexin V ) in flow to be
compared with chromium release ?
What E:T ratios to start with ( more or less the same as in Chromium release ?)
What about incubation time ?
Thanks for any suggestions !
HP
*******************************************
hpsteff@mzdmza.zdv.uni-mainz.de
Hans-Peter Steffens
http://vzdmzi.zdv.uni-mainz.de/~hpsteff/virology.html
Institute for Virology, Johannes Gutenberg-University Mainz
Obere Zahlbacher Strasse 67, 55101 MAINZ, Germany
Tel.: ++49-6131-17-3165
Fax: ++49-6131-39-5604
*******************************************
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web