THP-1's and fluorescent dyes

mckenziekroeger.jl@pg.com
16 Dec 96 18:08:00 -0500

Has anyone worked with the THP-1 cell line using Dihydrorhodamine 123 (DHR)
or Hydroethidine (HE)? I am in search of a method to stimulate these cells
with LPS, an inflammatory cytokine, or virus, and measure oxidative burst.
As of yet, my efforts have come up empty, but I thought there may be some
unpublished or hard to find methods out there in Flowland. Any help,
insights or watch-outs will be appreciated.

Thanks...Jodi McKenzie-Kroeger


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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu