Re: FACScan side scatter problems
Larry Arnold (lwarma@med.unc.edu)
Tue, 26 Dec 95 11:02:56 EST
In regards to the discussion of FACScan side scatter problems, we have not
seen the problem which has been described by "y'all" on our FACScan which
was been in operation for 3+ years. We do not have any special electrical
supply connections and it seems unlikely that this is the problem in so many
different sites. However, it would always be a good idea to provide some
sort of electrical surge/loss protection for the computer. We have installed
an UPS(uninterupptable power supply) from APC. We do not use the B-D
computer for data acquisition but instead use the Cytomation system. The
Cytomation system takes off the signals prior to the FACScan ADCs so it is
using very little of the FACScan circuitry. I doubt that the problem is air
bubbles. Usually when we think we have air bubbles the signals in all
channels degrades. It sound like B-D service needs to get involved to solve
this problem.
Larry Arnold
UNC Flow Cytometry Facility
Chapel Hill
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu