RE: CD57+ lymphocytes
kukuruga%kasle1.dnet.wayne.edu@rocdec.roc.wayne.edu
Fri, 26 Jan 1996 13:54:54 -0500
reply to Pavlick...Obviously, the "dim" cells have too few binding sites to
accumulkate enough AV-magbeads to effectively select them from the negative
cells. Since what you really need is more biotin to bind the AV-beads, have
you tried a sandwich. Adding free AV, then biotin, THEN following with
your AV-beads?
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
