Re[2]: apoptosis antibodies (FASL, GRANZ, PERFOR)

tom_frey@bdis.com
Fri, 13 Dec 96 16:53:31

Andreas Christaras

Thanks for providing the information below regarding your experience with anti-
fas-ligand mAbs. However, I had the impression (possibly mistaken) that at
least one of these mAbs recognized an intracellular epitope. Could you clarify
which are able to stain cells without premeabilization?

Tom_Frey@bdis.com

Dear Maria,

following companies provide antibodies against FAS-L (CD95L)

Transduction Laboratories http://translab.com clone 33
in my experience on flow cytometry this mAb works fine
available in purified unlabelled format

PharMingen http://www.pharmingen.com clone NOK-1
this is the antibody I regularly use and it works perfectly
available in purified unlabelled format and biotinylated

PharMingen http://www.pharmingen.com clone NOK-2
I have no experience using this antibody
available in purified unlabelled format

Hope this helps a bit

Andreas Christaras
Heinrich-Heine University Children's Hospital
Laboratory of Experimental Hematology and Stem Cell Transplanatation
KMT-Labor Building 14.82
Moorenstrasse 5
40225 DUESSELDORF
Germany

FON +49-211-81-16103 FAX +49-211-81-16191
E-M christar@uni-duesseldorf.de


Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu