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Santa Cruz Biotechnology (CA): Cat# sc-834; rabbit polyclonal; reacts w/
mouse, rat human fas-l; recommended for western or immuno, though there
is a group that used it for flow (see PNAS 1996May28;93(11):5533-8).
Pharmingen (San Diego, CA): many fas-l antibodies available, and some
have been tested for flow, but be aware of the metalloprotease inhibitor
treatment issue. Pharmingen can tell you more about it.
You can also try Alexis Corp. in San Diego, whose anti fas-l has been
used for immuno (see Science vol.274, 11/22/96, 1363-6). I believe it
has also been tested for flow.
Hope this helps-
Amy Wagelie-Steffen
LAD, NIH
>----------
>From: Maria Cristina Ribeiro de Castro[SMTP:mcrc@usp.br]
>Sent: Thursday, December 05, 1996 9:41AM
>To: Cytometry Mailing List
>Subject: apoptosis
>
>
>
>
> I have been looking for gramzyme, perforin, Fas-ligand
>antibodies for immunohistochemistry and FACS analysis in kidney, blood
>and urine samples. Does anybody know which company are producing them?
>Thanks in advance.
>
>
>Castro, MCR
>Sao Paulo University
>Brazil
>
>
>
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web