The easiest way to measure these 3 parameters is to stain for DNA content
which allows you to quantitate apoptosis [below diploid] and proliferation
[above diploid] simultaneously. If you wish to analyze cell death using a
different method you have many choices, including TUNEL, annexin, which are
in kit form. There are also many other methods discussed in current
literature.
Good luck,
Tom
--------------------------------------------------------
Thomas W. Mc Closkey, Ph. D.
Director, Flow Cytometry
North Shore University Hospital
Biomedical Research Center
350 Community Drive
Manhasset, Long Island, New York 11030
ph: 516-562-4844 [office]; 516-562-1135/4641 [lab]
4/23/97 11:37:08 AM
E-mail: thomasm@nshs.edu
--------------------------------------------------------
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web