More thoughts on FACScan FLx drift

Geoffrey Osborne (Geoff.Osborne@anu.edu.au)
Wed, 4 Dec 1996 09:19:59 +1100 (EST)

Eric,
Another possible cause of the FLx drift your experiencing can be a
partially pinched waste line on the FACScan. We use a "home made" large tank
system for sheath and waste, and can replicate the drift symptoms you
describe if we have the lower front panel door of the instrument open, such
that it squeezes the sample waste line which normally flow underneath it.
Obviously, this normally won't occur on an unmodified instrument, however it
may be worth checking the waste tank lines anyway. This drift problem on our
system has normally only be noticed when the instrument is being serviced,(
panels opens,) and small calibration beads run in a linear mode, a scenerio
not unlike your cell cycle analysis using PI.
The only other comment I'd make is that we find Xtran from BDH
chemicals, particularly good for cleaning the sample probe up when 7AAD or
PI have been used.

Geoff
======================================================================
Geoffrey Osborne | ____ __ o Ahh!
Flow Cytometry (FACS LAB) | __ `\ <,_
John Curtin School of Medical Research, | __ (*)/ (*)
Australian National University, | ==============|
CANBERRA, AUSTRALIA. | |--|
Email: Geoff.Osborne@anu.edu.au | |--|...
Phone: 61 6 249 3694
FAX: 61 6 249 2595
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