Re: negative control for flow crossmatch

Karen Henell (henellk@ohsu.edu)
Wed, 29 Jan 1997 13:07:46 -0800

Austin Lin inquired about negative control serum for use in flow crossmatching
for transplantation. Here is a reply from my colleague:

We, at the Lab of immunogenetics and Transplantion at Oregon Health Sciences
University, use a single donor blood group "AB" male serum as our negative
control. We recalcified a unit of AB plasma from our local blood bank, and have
found it to be very reliable over the 3 years that we have used it.

We found that the pooled negative control products gave us too much variability.

If you have any more questions, feel free to write to me directly.

Mary Ann Head
Oregon Health Sciences University
headm@ohsu.edu


Home Page Table of Contents Sponsors E-Mail Archive Web Sites

CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu