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Dear Dr Mann:
This is my 1st time answering a question on the net. I usually just
read the chat. Over the years I have used CD103 and find that it is
expressed in all kinds of chronic B-cell leukemias, particularly the
CD11c only variety ( ie SLVL, marginal zone LPD, leukemic Maltomas
etc).. I would not make a diagnosis of HCL with TRAP negativity and
absent CD25 and 11c. I have seen several hundred cases of HCL over the
last 8-10 years, but none without CD11c and CD25. Most also express
CD103. However, CD103 is certainly not specific for HCL. I have found
CD103 expressed in a number of so-called HCL look- alikes. What about
monocytopenia and/ or BM histology. What do the cells in the PB look
like? HCL has a characteristic nuclear morphology.
The more difficult problem are the cases of CD11c only disease that
lack CD25, usually TRAP negative, but may express CD103. Are these
HCL-variants or something else?
Randy Gascoyne
Hematopathologist
=09
=09
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=BBFrom: Kees Pot
=BBTo: Randy Gascoyne
=BBSubject: FW: hairy cell leukemia
=BBDate: Tuesday, 31 December, 1996 9:21AM
=BB
=BB
=BB ----------
=BBFrom: mann0002@mc.duke.edu
=BBTo: Cytometry Mailing List
=BBSubject: hairy cell leukemia
=BBDate: Tuesday, December 31, 1996 6:36AM
=BB
=BB
=BBI received peripheral blood for immunophenotyping for a question of
hairy cell
=BBleukemia. (The patient is an elderly male with splenomegaly and
circulating
=BB"hairy cells".) There is bright expression of the pan B cell markers
CD19, CD20
=BBand CD22 as well as lambda light chain. The cells are negative for =
CD5
and CD10.
=BBAlthough there is expression of CD103, the cells are negative for =
CD11c
and
=BBCD25. The cells are reportedly TRAP negative.
=BB
=BBI know that some people consider staining for CD103 highly specific =
for
hairy
=BBcells, but the absence of CD11c is very worrisome to me. What other B
cell
=BBneoplasms have you found to be CD103 positive? Would anyone consider =
a
=BBdiagnosis of hairy cell leukemia in the absence of staining for =
CD11c.
=BB
=BBKaren Mann
=BB
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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