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1. We have had a couple of these cases, the immunophenotype is more
consistent with a peripheral T cell lymphoma (can also have a lymphoblastic
cytology) than with T- precursor ALL. These lymphomas are more common in
young adults than in children, so the age of the patient fits very well.
2. The ALCL are very difficult to get in suspension and run flow. We do
immunophenotyping on tissue sections instead.
Best wishes
Anna
Anna Porwit-MacDonald
Haematopathology Lab.
Department of Pathology,
Karolinska Hospital
Stockholm
anpo@mb.ks.se
>We recently ran phenotyping on bone marrow cells from a 21-year old patient
>having cells which appeared morphologically to be lymphoblasts. The cells
>were CD2+, CD3+, CD7+, CD45+, DR-, CD5- and Tdt-. This is the first case we
>have seen of Tdt negative lymphoblasts and were wondering how often this has
>been observed by others. Our cases of pediatric ALLs are usually CD45- and
>Tdt+, suggesting that our current case may have a slightly more mature
>phenotype although presenting clinically as an ALL.
>
>A second unrelated question is how to achieve maximal viable cell recovery on
>anaplastic large cell lymphomas. Although we usually recover sufficient
>numbers of viable lymphoid cells on fresh lymph node biopsies, we
>occasionally will have a case in which the cell recovery is extremely low
>even though the original tissue sample appears adequate. We routinely gently
>mince the tissues on a fine mesh screen and wash the cells with HBSS before
>staining. Any contaminating red cells are lysed with ammonium chloride before
>staining. Have others run into problems with lymphoma cells that are
>extremely fragile? Thank you in advance for your suggestions.
>
>Lucy Kimura
>Department of Pathology
>Tripler Army Medical Center
>TAMC, Hawaii 96859-5000
>Lucille_H.Kimura@TAMC.CHCS.AMEDD.ARMY.MIL
>
>
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
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If you have any comments please direct them to
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