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I believe you are speaking about our ModFit LT 1.01 which is packaged
with Becton Dickinson's CellQuest workstations. As you point out it
does not handle synchronized cell populations well, nor-was it designed
for this type of analysis. You will be happy to learn that we have just
released ModFit LT 2.0 which contains a Synchronization Wizard. This
tool enables users to perform this type of analysis with unprecedented
ease. I would be happy to have a demo disk sent to you for evaluation.
You will be happy to learn as well that there is an upgrade path to the
new version. We sent notices out to all of the people who sent in their
registration cards but yours must have been lost in the mail.
In any case you should contact your BD rep and inquire about the
upgrade. If you provide me with your address and FAX, I will FAX you
some information immediately. You can also see the new features on our
WEB site (www.vsh.com).
If I can be of any further assistance please do not hesitate to contact
me.
Best regards
Don Herbert
Verity Software House, Inc.
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From: austin[SMTP:austin63@ms12.hinet.net]
Sent: Friday, October 28, 1994 11:58 AM
To: cyto-inbox
Subject: RE:Modfit S/W
Dear Flow-ers,
I need some advices regarding the use of ModFit S/W.
A colleagues of mine is interested in studying the effect of various
anti-sense oligo's on cell cycle progression of synchronized HeLa cells.
He have tried to use BD's ModFit s/w to analyze the data and did not
get good reports with some of the data files. I studied some of the
graphs and found that bad reports are usually derived from
non-standarized cell cycle distribution. For example, one set of data
can have 10% G0/G1, 80% S and 10% G2/M.
The s/w scans three peaks and take the second S peak as G2/M peak.
Another example will be a set of data with 20% G0/G1 and 80% S and
invisible G2/M. The s/w will scan and see only one peak.
I used to work with the earlier vesion (CellFit) and my only
experience with ModFit is with standard cell cycle data. It appears that
the CellFit S/W does a better job in these type of analysis. I had
used CellFit for similar type of analysis and it generated better
reports.
We would like to know if ModFit is fitted for such an analysis, and if
yes, how to get around the proplems we had? We would try other s/w if
modfit is not adequate for such analysis. Would anyone suggest a good
Mac-s/w for us? (We have a FACScan and Macintosh Quadra 650) Thank you
in advance.
Regards
Austin Lin
austin63@ms12.hinet.net
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web