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According to the paper by Doornbos et al. (Cytometry 15, pp. 267-271
(1994) the TO-PRO-3 dye may be useful to resolve cell cycle
distributions by flow cytometry. The TO-PRO-3 dye is not membrane
permeant. If you are going to use this dye in conjunction with
antibodies you may have to tinker around with cell fixation /
permeabilization procedures. I am not sure that TO-PRO-3 is specific
for DNA: maybe you need to digest RNA in order to get a sharp CV.
Perhaps Harry Crissman (Los Alamos) has some useful experience with
this dye.
Good Luck!
Martin Poot
Molecular Probes, Inc.
Flow Cytometry Group
P.O. Box 22010
EUGENE OR 97402
Tel: (541) 465-8300
FAX: (541) 344-6504
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web