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Hi everybody,
I am doing some staining for IL-2R and got surprised to see that, in spite
of having many proliferating T-cells (as assessed by 7-AAD), the percents of
IL-2R positive cells are low. Comments? Thank you very much for your attention.
Activated, proliferating cells can shed IL-2R. An example is various forms of lymphocytic
alveolitis, where the percentage of alveolar T cells expressing IL-2R is low, but soluble IL-2R
may be detectable in the BAL. You don't give any more details of your system or what cells
you are looking at, but maybe you could look at soluble IL2-R.
Dr. D O Donnell,
University College Dublin
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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