Re: Macrophage membrane marker for counting % Macs in a
Howard Shapiro (hms@shapirolab.com)
Tue, 4 Mar 1997 09:18:21 -0500 (EST)
Monocytes in peripheral blood are relatively well differentiated from lymphs
and granulocytes by the combination of forward and orthogonal scatter
measurements. At least in mammals, cells of the monocyte/macrophage lineage
contain esterases not present in lymphs and granulocytes; Technicon's
original Hemalog D flow cytometric differential counter (1972) used a
colorogenic esterase substrate for monocyte counting. However, the reaction
product was detected by absorption and scattering. Years ago, I tried
fluorescein dicaproate as a fluorogenic substrate for esterase, and I seem
to remember it working well enough to discriminate monocytes from other
peripheral blood leukocytes. I believe the material came from Research
Organics; if they don't have it, they may have another fluorogenic substrate
for esterase. Given the number of different species in which you need to
count macrophages, it is unlikely that you would find suitable antibodies
for all of them, and even less likely that you'd find one which detected
macs across broad species barriers, so scatter and esterase are probably
your best bets.
-Howard
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