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Regarding your question about the lack of deletion of certain Vb subsets:
Although I haven't done these experiments myself, I looked into doing them
in some of our transgenic mice that are on the C57BL/6 background. What I
found was that the evidence for an increase in certain TCR Vb subsets after
injection of superantigen came from studies primarily done in BALB/c mice.
I have read one paper (Immunity 2:185-194, 1995) where they used both
C57BL/6 mice and BALB/c mice. They saw no expansion in CD4+ Vb8+ T cells on
day 4 after injection of SEB into B6 or (B6 X 129)F2 mice. I think these
changes may be dependent on the strain of mice that you use. Some
superantigens preferentially bind to I-E, and B6 mice do not express I-E.
I hope this helps,
Charles Sentman
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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