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>In the course of some R&D work on apoptosis, we "accidently" came out with
a
>good method to evaluate the Fas receptor occupancy in a highly
quantitative
>and very standardized manner.
>What would be the application(s) of such an approach?
The ability to define those Fas expressing cells already triggered
by Fas ligand is potentially very important in the context of HIV infection
as well as certain autoimmune disorders. Although other regulatory
molecules are definitely involved [bcl-2 family, etc], the Fas/Fas L
interaction may be a requisite step for peripheral T cell deletion. For
example, PBMC from HIV infected and uninfected individual s both express
Fas, but only the cells from infected people undergo rapid in vitro
apoptosis-presumably triggered in vivo. So an application might be to
quantitate the % of cells with Fas R already occupied.
Regards,
Tom
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Thomas W. Mc Closkey, Ph. D.
Director, Flow Cytometry
North Shore University Hospital
Biomedical Research Center
350 Community Drive
Manhasset, Long Island, New York 11030
ph: 516-562-4844 [office]; 516-562-1135/4641 [lab]
6/10/97 1:01:25 PM
E-mail: thomasm@nshs.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web