Re: HLA TYPING B27 BY FLOW

Jorge Neumann (neumann@santacasa.tche.br)
Thu, 17 Apr 1997 11:33:23 -0300 (EST)

Yes, they agree with it. I have complained to One Lambda about
this inespecificity of their anti B27 and the answer was that their
antibody cross react with B44, B17 (particularly B57) and B16 (B38 and
B39). They claim that a new, diluted version, of the monoclonal is able to
solve this problem. I have not tried it so far.

Jorge Neumann
Transplantation Immunology Lab
Santa Casa Hospital
Porto Alegre, Brasil

On Tue, 15 Apr 1997, ernest stapleton wrote:

>
> Our laboratory has also evaluated B27 typing by flow and have found
> there is no monospecific antibody to B27 yet. The one lambda clone
> also reacts with most hla B44 positives as well. Other labs have also
> found this cross reaction.
> If any lab does screen for B27 there are other antibodies available
> (they also cross react with B7), namely Serotec and Becton Dickinson.
> I would STRONGLY recommend ANY positives, weak or strong, to be
> confirmed by serology or molecular methods before reporting it as
> such.
> Does One lambda admit to this cross reactivity to B44 yet? It has been
> confirmed by our lab and sylvia wu's lab in british columbia but yet
> to see it in their literature.
>
> Ernest Stapleton
> Clinical Immunology Lab
> St. John's NFLD
>


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