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Imagine staining human PBMCs with FITC-CD4 and PE-CD8. You could sort
FITC+/PE- to the left and FITC-/PE+ to the right. One might think that
collecting the center stream would be a good way to recover the B
lymphocytes (CD4-/CD8-). If all goes well, all of the B cells will be
in the center stream, but they will be in small proportion to all of the
particles/cells mentioned in the paragraph above. Furthermore, the
dilution factor of the center stream is much, much higher than that of
the side streams.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web