response to :sheath fluid
KUKURUGA@medmail.med.umich.edu
Mon, 27 Jan 1997 16:16:46 -0500 (EST)
I've used water as a sheath in a FACS440, and also in a FACScan. It had
no effect on immunofluorescence, although I noted a slight increase in
% c.v. for DNA histograms -- usually only a fraction of a percent.
Obviously, one cannot use water as sheath in a charge-based sorter
(I have used as little as 0.009% saline and still gotten reasonable
droplet charging, however).
One drawback might be that the possibility of back-flow into the sample,
thus changing osmolality and possibly affecting viable cells adversely,
assuming one runs cells viably.
I currently run an isotonic diluent from a commercial source ($13 / 20 liter),
-- I filter it on-site through a 0.2um cartridge before using -- in my Coulter
sorters and my XL. Haven't had a need to try water.
I suppost a good question might be . . . why do you want to use water?
MAK.
Mark A. KuKuruga
University of Michigan Flow Cytometry
kukuruga@medmail.med.umich.edu
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
