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There are 4 things to control when sorting - drop drive frequency,
drop drive amplitude, delay time for drop charge, and drop charge
phase (wrt drop drive).
>From the brief information you have given, it appears that your
sorted stream is "fanning" - that is, all sorted drops for a single
event are not getting equivalent charge. You can check if this is so
by viewing the deflected stream during a test sort - backlighting is
best. The fanning can be minimized/eliminated by adjusting the
phase.
Good luck!
-Marty Bigos
Stanford Shared FACS Facility
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web